The GeneTex SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA kit is an in vitro tool that quantitatively detects the SARS-CoV-2 Spike receptor-binding domains (RBDs) of the "wildtype" SARS-CoV-2 strain as well as those of the Alpha, Beta, Gamma, Delta, Alpha (E484K), and Kappa variants. This assay employs a pair of monoclonal antibodies specific for the SARS-CoV-2 RBD, with the lowest detectable dose concentration of standard RBD protein being 1.8 pg/ml (Alpha variant).
APPLICATION
Application Note
The suitable sample types include cell culture supernatant, plasma and serum. The suggested sample loading amount is 50μl per well. Please see the protocol for detailed procedure. Assay range : Please refer to the protocol.
Positive Control
S1 Standard (lyophilized recombinant S1 protein)
Product Note
This ELISA kit detects Spike RBD proteins derived from multiple SARS-CoV-2 VOCs, including Omicron variant (BA.1 and BA.2).
PROPERTIES
Storage
The entire kit can be stored at 2-8ºC for up to 6 months.
Conjugation
Horseradish peroxidase(HRP)
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Sandwich ELISA detection of recombinant Spike ECD proteins derived from different strains of SARS-CoV-2 virus (ie., wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit (GTX536267).
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GTX536267 ELISA Image
Sandwich ELISA detection of recombinant Spike RBD proteins derived from different strains of SARS-CoV-2 (ie., wild type; BA.1 Omicron variant; BA.2 Omicron variant; BA.4 / BA.5 Omicron variant; BF.7 Omicron variant; BQ.1 Omicron variant; XBB Omicron variant) using SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit (GTX536267).
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GTX536267 ELISA Image
Sandwich ELISA detection of recombinant Spike RBD proteins derived from different strains of SARS-CoV-2 (ie., wild type; B.1.1.7 Alpha variant; B.1.351 Beta variant; P.1 Gamma variant; B1.617.2 Delta variant, B.1.617.1 Kappa variant; BA.1 Omicron variant; BA.2 Omicron variant; B.1.1.7 Alpha variant with E484K) using SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit (GTX536267).
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GTX536267 ELISA Image
Sandwich ELISA detection of recombinant Spike ECD protein (GTX135972-pro) derived from wild-type strain of SARS-CoV-2 using SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit (GTX536267).
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GTX536267 ELISA Image
ELISA analysis of SARS-CoV-2 (COVID-19) Spike protein S1 subunit using SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit (GTX536267).
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GTX536267 ELISA Image
ELISA assay using lysates of 293T cells overexpressing the spike proteins from SARS-CoV-2, SARS-CoV, or MERS-CoV. The SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit (GTX536267) specifically detects only SARS-CoV-2 spike protein.
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GTX536267 ELISA Image
A. Recovery assay was determined by spiking SARS-CoV-2 spike and RBD proteins at various levels throughout the assay range in the listed samples. B. To determine linearity, the listed samples were spiked with SARS-CoV-2 spike and RBD proteins and diluted with Assay Diluent in 1:2 and 1:4. The value was assessed by comparing it to undiluted samples.
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